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文献信息

Phytochemical profile, chemotaxonomic studies and in vitro antioxidant activities of two endemisms from Madeira archipelago: Melanoselinum decipiens and Monizia edulis (Apiaceae)

期刊名:Chemistry & Biodiversity
文献编号:
文献地址: http://onlinelibrary.wiley.com/doi/10.1002/cbdv.201600039/abstract
发表日期:

Melanoselinum decipiens and Monizia edulis (Apiaceae) are two endemic plants from Madeira archipelago which phytochemical composition remains little explored, despite their use in folk medicine. Using liquid chromatography with diode array and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MSn) analysis, their polyphenolic profile was established for the first time. Fifty six compounds were identified, 5-O-caffeoylquinic acid, quercetin-O-(malonyl)hexoside, luteolin diacetyl and quercetin-O-hexoside being the major constituents in the leaves of both plant species (≥ 0.76 mg g-1 of dry extract). Principal component analysis (PCA) provided a suitable tool to differentiate targeted plants. Naringenin-6,8-di-C-glucoside, quercetin 3-O-pentosylhexoside and 1,5-O-dicaffeoylquinic can be used as discriminatory taxonomic/geographical markers for M. edulis subspecies from Madeira and Porto Santo populations. This methodology of using polyphenols as chemotaxonomic markers proved to be useful for identification of plant species since the results are consistent with previous taxonomical data.

The free radical scavenging activities of the M. decipiens extracts proved to be higher than those of M. edulis, which correlated well with their phenolic content (R2 > 0.906).


... Extrasynthese (Genay, France). 1,5-O-dicaffeoylquinic (99.2%), 3,4-O-dicaffeoylquinic (99.0%), 3,5-O-dicaffeoylquinic (99.2%) and 4,5-O-dicaffeoylquinic (99.3%) acids were purchased from Biopurify Phytochemicals Ltd. (Chengdu, China). LC–MS n grade acetonitrile (CH3CN) ...