我的账户 7×24小时客服热线:400-829-7929 语言:
热门产品: 人参皂苷Rh2,人参皂苷Rg3,胡萝卜苷, 木犀草苷
产品分类
在线咨询
联系电话:
销售:
400-829-7929(7*24小时)
028-82633860
028-82633397 
028-
82633165
技术服务和产品定制:
028-82633987
在线服务:  
沈帅 
文静  点击这里给我发消息
贺丹丹  
点击这里给我发消息
      
文献信息

Thai perilla (Perilla frutescens) leaf extract inhibits human breast cancer invasion and migration.

期刊名:Maejo International Journal of Science and Technology
文献编号:
文献地址: http://www.mijst.mju.ac.th/vol12/112-123.pdf
发表日期:2018, 12(02)
Abstract: Thai perilla (Perilla frutescens), also called Nga-mon, contains a substantial quantity of bioactive substances including phenolics and flavonoids. These phytochemicals have been linked to various bioactivities of P. frutescens such as in vitro and in vivo antiinflammatory, anti-oxidative and anti-cancer capacities. In this study we evaluated antiinvasive and anti-migratory activities of Thai perilla leaf extract (PLE) on human breast cancer cells, MDA-MB-231. Our results demonstrate that rosmarinic acid is the main constituent of PLE. In vitro cytotoxicity analysis shows that PLE, at 24-hr exposure, is not toxic to MDA-MB-231 cells. A Boyden chamber-based transmembrane assay shows that PLE at a non-toxic dose (12.5-50 µg/mL) dramatically exhibits an inhibitory effect on cell invasion and migration. Gelatin zymography shows that PLE at a concentration of 100–400 µg/mL dose-dependently decreases matrix metalloproteinase-9 (MMP-9) secretion (p < 0.05–0.001) and activity (p < 0.001). Our data indicate that PLE can inhibit breast cancer cell invasion and migration through the reduction in activity and availability of MMP-9. Our observations also suggest that rosmarinic acid in PLE may account for the anti-invasion and anti-migration activities. In particular, rosmarinic acid as a food-derived chemotherapeutic agent can potentially be used in cancer chemotherapy.

… [24] with modifications. Apigeninluteolin and rosmarinic acid (Biopurify, China) were used as standards. The PLE and standards were loaded onto a C18-EPS Rocket column (53 mm x 7 mm, GRACE). The isocratic elution was carried out for 5 min …