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文献信息

Validated high performance liquid chromatography for simultaneous determination of stability of madecassoside and asiaticoside in film forming polymeric dispersions

期刊名:Revista Brasileira de Farmacognosia
文献编号:
文献地址: https://www.sciencedirect.com/science/article/pii/S0102695X18300760
发表日期: May–June 2018
Abstract

The objective of the work was to validate the high performance liquid chromatography for simultaneous determination of stability of madecassoside and asiaticoside in Centella asiatica (L.) Urb., Apiaceae, extract-loaded film forming polymeric dispersions. High performance liquid chromatography method was validated in five topics: linearity and range, limit of detection and limit of quantitation, specificity, precision, and accuracy. Results showed the method had a good linearity (R2 > 0.9990) in the range of 5–150 μg/ml and specific. The limit of detection and limit of quantitation of madecassoside were 81 and 245 ng/ml and asiaticoside were 21 and 64 ng/ml, respectively. The percent relative standard deviation of intraday and interday precision were less than 1 and 3%, respectively. The accuracy presented as percent recovery was 101.54–103.29% for madecassoside and 100.39–102.58% for asiaticoside. This validated high performance liquid chromatography method was used to determine the stability of the formulation containing Centella asiatica extract. Centella asiatica extract-loaded film forming polymeric dispersions used Eudragit® RS 30D and Eudragit® RL 30D as film former, glycerin as plasticizer, and absolute ethanol as solvent and penetration enhancer. Three formulations with different ratio of Eudragit® RS 30D and Eudragit® RL 30D were prepared and stored for 90 days at 4 °C, 25 °C, and 40 °C. Stability results showed that almost all of the formulations were unstable at 25 °C and 40 °C. Except, two of three formulations were stable at 4 °C. However, the formulation was further developed to improve the stability of madecassoside and asiaticoside in the formulation.

… Materials and methods. Chemicals and reagents. Madecassoside (purity 95.0%, HPLC) was purchased from Sigma–Aldrich Inc., Missouri, USA. Asiaticoside (purity 99.81%, HPLC) was purchased from Chengdu Biopurify Phytochemicals Ltd., Sichuan, China … 
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