A method for the ecologically pure extraction of chlorogenic acid from the marsh cinquefoil in a medium of subcritical water has been developed. The content of chlorogenic acid in extracts obtained in subcritical water and in an aqueous ethanol solution has been determined by HPLC. A high efficiency of extraction of chlorogenic acid from marsh cinquefoil tap roots in subcritical water without using expensive organic solvents has been demonstrated.
The isomer of 3O caffeoylquinic acid (99.1%; Chengdu Biopurify Phytochemicals Ltd., CAS no. 327 97 9; Catalogue no. C07007) served as a standard.
