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文献信息
LC–MS Characterisation and Quantification of Known and Unknown (poly)phenol metabolites — Possible Pitfalls and their Avoidance
This review focusses on the LC–MS characterisation and quantification of dietary (poly)phenols and their metabolites. It draws attention to errors, omissions, and misunderstandings that appear frequently in published papers, and suggests strategies for their avoidance. Aspects covered include the use of authentic standards and surrogate reference materials, the importance of collecting and archiving Total Ion Current MS data, the limitations of using on-line compilations of accurate mass MS data to assign unknown components when multiple isomers are possible, and the often understated magnitude of person-to-person variation that may significantly impact at population level any potential health benefit.
