我的账户 7×24小时客服热线:400-829-7929 语言:
热门产品: 人参皂苷Rh2,人参皂苷Rg3,胡萝卜苷, 木犀草苷
产品分类
在线咨询
联系电话:
销售:
400-829-7929(7*24小时)
028-82633860
028-82633397 
028-
82633165
技术服务和产品定制:
028-82633987
在线服务:  
沈帅 
文静  点击这里给我发消息
贺丹丹  
点击这里给我发消息
      
文献信息

Transcriptomics and metabolomics reveal the cardioprotective effect of Compound Danshen tablet on isoproterenol-induced myocardial injury in high-fat-diet fed mice

期刊名:Journal of Ethnopharmacology
文献编号:
文献地址: https://www.sciencedirect.com/science/article/pii/S0378874119327564
发表日期:10 January 2020
Abstract

Ethnopharmacological relevance

Compound Danshen tablet, an herbal preparation consisting of salviae miltiorrhizae, notoginseng and borneolum, is extensively employed clinically to treat angina pectoris, coronary arteriosclerosis and significantly improve microcirculation.

Aim of the study

To reveal the potential underlying cardioprotective mechanism(s) in isoproterenol-induced myocardial injury in high-fat-diet fed mice.

Materials and methods

Cardiac transcriptomics was analyzed by Illumina mRNA-Seq sequencing. The restored cardiovascular diseases (CVD)-related genes by Compound Danshen tablet were validated by quantitative real time polymerase chain reaction (qRT-PCR). Furthermore, Cardiac metabolomics were also performed using gas chromatography-mass spectrometry.

Results

From the transcriptomics study, we found the levels of 24 up-regulated and 44 down-regulated genes in the control compared to model groups. Among them, seven gene levels were restored by treatment of Compound Danshen tablet. Four CVD-related genes at the mRNA level (Sprr1a, Ppp1r3c, Bmp10 and Hspa1b) were validated successfully by qRT-PCR. From the metabolomics study, 37 differentially expressed metabolites were identified between the control and model groups. Among them, 21 metabolites were restored by treatment of Compound Danshen tablet. These altered metabolites are involved in glucose metabolism, fatty acid metabolism and amino acid metabolism.

Conclusion

These genes and metabolites might provide clues for further molecular mechanistic study of Compound Danshen tablet.

… ginsenoside Reginsenoside Rb1notoginsenoside R2ginsenoside Rg2,
ginsenoside Rh1ginsenoside Rdnotoginsenoside Feginsenoside Rc,
ginsenoside Rb3notoginsenoside Fc and notoginsenoside Fa were from Biopurify